DNA

Part:BBa_K2995002:Design

Designed by: Clara Fikry   Group: iGEM19_Waterloo   (2019-10-16)


CcaS/R inducible MetE cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1835
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2638


Design Notes

This part was integrated into the genome of E. coli.


Source

MetE cassette from pSKA397 (https://www.addgene.org/80380/)

References